Share this content on Facebook!
07 Jul 2015

High Performance Liquid Chromatography (HPLC)

High Efficiency Liquid Chromatography (HPLC) is an analytical tool that separates, identifies and quantifies elements in an example. It is a commonly used program in analytical chemistry and biochemistry fields. Basically, the device carries the sample employing a solvent or combination of solvents to the immobile phase, where separation of compounds happens. A detector captures the separated compounds and signals are delivered to the integrator to create a graphic visual. Lc Autosampler

HPLC consists of the components below:

-- Cellular Phase - here is usually or the solvent a blend of solvents used to transport the samples through the complete system. The solvents must be miscible in the mixture; else the solvents can cause cause pressure build-up in the HPLC program. The ratios of each and every solvent component in the cellular phase affect the separation of compounds as well as investigation length.

-- Pump or solvent delivery device - this component is to deliver samples and the mobile phase throughout the system at a continuous flow rate or stress. Usually, for analytic purposes, HPLC pump is defined to operate at constant flow rate.

-- Injector Port or auto sampler - analytical samples are introduced by means of this component. Samples added through injector interface have to be manually injected utilizing HPLC syringes that are appropriate. Vehicle sampler enables an analyst to load all the samples in the HPLC system along with the program will automatically select the right sample to inject at preset states.

-- Still phase - also known as column. This area of the system is in fact the heart of separation. It's made from tightly packed material in a metal column. Due to the compactness of the packed material, high pressure are required to pump or deliver dissolving agents through the system, hence HPLC sometimes are phrase as High-Pressure Fluid Chromatography. As the samples flow-through the column, the compounds in the sample will interact simultaneously using the mobile and fixed phase in a different manner to afford different elution time of each compound. The goal of every evaluation would be to separate the peak of curiosity from other existing compounds.

-- Detector - this unit finds the detached compounds in the sample. There are various sensors using distinct style of detection such as ultraviolet, fluorescence, mass spectroscopy and refractive index. Hplc Autosampler

-- Integrator - integrator turns the signals conveyed from the detector into visual output called chromatograms. Nowadays integrators come in the form of computer systems as opposed to the conventional ones which use paper charts.

Justine Choy has obtained a Bachelor Degree with Honours in Chemistry and has completed her Master's Degree in the field of Pharmaceutical Technology. Currently, she is holding a post in an R&D lab of a nearby pharmaceutical organization.


There isn't any comment in this page yet!

Do you want to be the first commenter?

New Comment

Full Name:
E-Mail Address:
Your website (if exists):
Your Comment:
Security code: